Atlanta United 2
Austin FC II
Carolina Core FC
Chattanooga FC
Chicago Fire FC II
Colorado Rapids 2
Columbus Crew 2
Crown Legacy Football Club
FC Cincinnati 2
Houston Dynamo 2
Huntsville City Football Club
Inter Miami CF II
Los Angeles Football Club 2
New England Revolution II
North Texas SC
NYCFC II
Orlando City B
Philadelphia Union II
Real Monarchs
Red Bull New York II
Sporting KC II
St Louis CITY2
Tacoma Defiance
Timbers2
The Town FC
Ventura County FC
Vancouver Whitecaps FC 2